Over the past twenty years, several techniques from biochemical and molecular genetics, such as enzyme electrophoresis and isoelectric focusing, have been widely and successfully applied to the study of population differentiation and evolution1. However, they have been less applicable to demographic problems such as assigning parentage to individuals within a population. This stems from a general weakness of data derived from enzyme loci: allele frequencies at polymorphic loci are sufficiently skewed that the majority of individuals are of one or two genotypes. Many enzyme systems can only be examined post mortem, so that the loci are of little use if the animals are to be studied in the wild. The search for new and more sensitive techniques for detecting genetic variation has continued, and recently a major discovery has come from molecular biology. Jeffreys et al.2 have reported the detection of a type of hypervariable 'minisatellite' DNA that is extraordinarily polymorphic in human populations. We have applied their technique to several bird species and particularly to a population of house sparrows (Passer domesticus) near Nottingham. We report here that one of the human minisatellite clones is a suitable probe for sparrow DNA and that it reveals variation as extensive as that found in man. These results suggest that analysis of minisatellite DNA will be a powerful tool in the study of demographic population genetics.
- 1. Lewontin, R. C. The Genetic Basis of Evolutionary Change (Columbia University Press, New York 1974). 2. Jeffreys, A. J., Wilson, V. & Thein, S. L. Nature 314, 67–73 (1985). 3. Burke, T. A. thesis, Univ. Nottingham (1984). 4. Jeffreys, A. J., Wilson, V. & Thein, S. L. Nature 316, 76–79 (1985). 5. Bulatova, N. S., Radjabu, S. I. & Panov, E. N. Experientia 28, 1369–1371 (1972). 6. Larson, R. A., Shode, R. O., Shapira, J. F. & Avise, J. C.J. molec. Evol. 17, 214–226 (1981). 7. Jeffreys, A. J. & Morton, D. M. An. Genet, (in the press).
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1988: published under
Cytogenetics and Cell Genetics
Vol. 47, No. 3, 1988
Article (PDF 1170 KB)
Original Article
DNA fingerprinting in domestic animals using four different minisatellite probes
M. Georgesa, A.-S. Lequarréa, M. Castellib, R. Hanseta, G. Vassartc
aChaire de Génétiquc, Faculté de Médecine Vétérinaire, Université de Liège, Bruxelles,
bLaboratoire de Démographie dcs Poissons et de Pisciculture Expérimentale, Service d’Ethologie et Aquarium, Faculté des Sciences, Université de Liège, Liège, and
cInstitut de Recherche Interdisciplinaire et Unité de Génétique Clinique et Moléculaire, Hôpital Erasme, Faculté de Médecine, Université Libre de Bruxelles
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